MAGTMLigA DNA Ligation Kit
The MAGTMLigA DNA Ligation Kit enables ligation of cohesive ends in 5 minutes at room temperature, and 5~15 minutes for
blunt ends. PCR product TA cloning takes only an hour of incubation.
Applications:
Suitable for cohesive or blunt ends, all common ligation, linker addition, TA cloning, library construction
- Features
• Speed : DNA ligation completes within 5~20 minutes at room temperature (cohesive or blunt end).
• Easy : Can be used for DNA ligation, Linker addition, TACloning, library construction.
• Complete Packaging : All buffers and enzymes needed are included, need not to prepare separately.
- Experimental Data
Figure 1. pGEM-7Zf (+) DNA were ligated with 300 bp, 600 bp and 700 bp DNA fragments by MAG LigA Kit.
Lane 1: Before ligation, pGEM-7Zf (+) DNA/EcoRIand 300 bp, 600 bpand 700 bp/EcoRIDNA fragments.
Lane 2: After 2 minutes at RT, then 70℃ 15 minutes inactivation.
Lane 3: After 5 minutes at RT, then 70℃ 15 minutes inactivation.
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