Plasmid Miniprep Purification Kit
The MAGSPIN-01 Plasmid Miniprep Purification Kit is based on a very simple and rapid procedure using spin column format to purify plasmid DNA from 1~10 ml liquid culture. The yield of up to 60 μg plasmid DNA is quantitative and reproducible, and the plasmid DNA quality is sufficient for direct use in any applications such as PCR, restriction digestion, cloning, automated DNA sequencing, southern blot, and in vitro transcription assay
- Features
Easy to use: Spin column format.
High purity: Purified DNA can be used for downstream applications such as automatic sequencing, in vitro transcription,
PCR and enzyme digestion.
High recovery: Each reaction can obtain 8~25 μg ultra pure plasmid DNA (up to 20 kb) from1~5 ml culture.
Fast:The procedures can be done in 10~15 minutes.
- Experimental Data
| Supplier | A260/A280 | Yield | Average |
| Supplier Q | 1.86 | 7.1 μg | 7.33 μg |
| 1.79 | 7.4 μg | ||
| 1.83 | 7.5 μg | ||
| Supplier Ga | 1.94 | 6.3 μg | 6.0 μg |
| 1.94 | 6.3 μg | ||
| 1.96 | 5.4 μg | ||
| APS LABS | 1.94 | 11.3 μg |
11.17 μg  |
| 1.97 | 10.8 μg | ||
| 1.94 | 11.4 μg |
Figure 1. Figure 1. Gel electrophoresis analysis of plasmid purified using plasmid purification kit from different suppliers.
Lane M: 100 bp DNA ladder
Lane Q: Supplier Q
Lane Ga: Domestic supplier Ga
Lane APS LABS: APS LABS
Figure 2.Sequencing result of plasmid DNA purified using APS LABS Plasmid DNA Purification Kit..
Detection signals are clear and easy to define, indicating plasmid DNA purified using APS LABS Plasmid
DNA Purification Kit is of high purity, and the buffer system does not affect subsequent applications.
Figure 3. Purification of pUC18 using 15 randomly chosen spin columns to test the stability of the kit.
Lane 1~15: pUC18 purified using 15 spin columns.
Lane M: APS-kb DNA Ladder
General Procedure
- DOWNLOAD PROTOCOL