TOPBlunt PCR Cloning Kit
TOPBlunt PCR Cloning Kit is suitable for cloning Pfu polymeraseamplifiedPCR products with blunt ends at room temperature in 5min. The vector provided in this kit contains topoisomerasecovalently coupled to its ends, which allows efficient cloning ofblunt-ended DNA fragments to the vector without using a regularligase.
- Features
Fast and Easy
• TOPO cloning system.
• Ligase not required, just 5 minutes to complete the ligation reaction.
• Uses ampicillin, blue/white screening for selection.
High Efficiency
• Insert size up to 3 kb.
• Contains restriction enzyme sites such as EcoR I for subcloning.
Figure 1. Colony PCR results from colonies carrying different sized inserts.
PCR products of 300 bp (50 ng/µl)、1 kb (50 ng/µl)、2 kb (100 ng/µl) and 3 kb (150 ng/µl) were ligated to TOPfast PCR Cloning Vectors, and colony PCR was performed using M13 primers.
For successful clones, the PCR result should be ~202 bp plus the insert size.
M : MAG-KB DNA Ladder
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