TOPfast PCR Cloning Kit
TOPfast PCR Cloning Kit is suitable for cloning Taq polymerase amplified PCR products (with A-tail overhangs) at room temperature in 5 min. The vector provided in this kit contains topoisomerase covalently coupled to its ends, which allows efficient ligation of DNA fragments with A-tail overhangs to the vector without using a regular ligase.
- Features
Fast and Easy
• TOPO cloning system.
• Ligase not required, just 5 minutes to complete the ligation reaction.
• Uses ampicillin, blue/white screening for selection.
High Efficiency
• Insert size up to 3 kb.
• Contains restriction enzyme sites such as EcoR I for subcloning.
Figure 1. Colony PCR results from colonies carrying different sized inserts.
PCR products of 300 bp (50 ng/µl)、1 kb (50 ng/µl) 、2 kb (100 ng/µl) and 3 kb (150 ng/µl) were ligated to TOPfast PCR Cloning Vectors, and colony PCR was performed using M13 primers.
For successful clones, the PCR result should be ~202 bp plus the insert size.
M : MAG-KB DNA Ladder
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