Tri-RNA Reagent
APSLABS Tri-RNAReagent is a reagent from the improved phenol and guanidine isothiocyanate (GSN) method for the single-step RNA isolation. During sample homogenization or lysis, Tri-RNA reagent maintains the integrity of the RNA, while disrupting cells and dissolving cell components. The composition of Tri-RNA reagent includes phenol and GSN in a mono-phase solution. A biological sample is homogenized or lysed in Tri-RNA reagent and homogenate is separated into the aqueous and organic phases by chloroform addition, vortexing,a nd centrifugation. RNA remains exclusively in the aqueous phase (clear upper phase), DNA in the interphase, and proteins remain in the organic phase (red color). RNA is precipitated from aqueous phase by addition of isopropanol and solubilized/concentrated. If needed, DNA and proteins can be sequentially precipitated from the interphase and organic phase with ethanol and isopropanol respectively, and solubilized/concentrated.
- Features
Single-step for the isolation of total RNA from tissues, cells, bacteria, plants, yeasts and biological fluids
The entire procedure for total RNA isolation is less than 1 hour.
The purified RNA can be applied in : RT-PCR, Northern hybridlation, RNase protection, Poly-A+RNA selection, Differential
display, and Micro-array assay.
- Experimental Data
Total RNA purified from human adipocytes using Tri-RNA reagent.
Total RNA purified from E. coli cells (3 samples) using Tri-RNA reagent.
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