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MAGTaq 5X Blood Direct PCR Master Mix Kit
The MAGTaq 5× Blood Direct PCR Master Mix Kit is designed for amplifying target DNA directly from whole blood, eliminating the need for lengthy DNA isolation process. The MAGTaq 5× Blood Direct PCR Master Mix Kit contains a unique blend of 5× concentrated mixture of all the essential components of a PCR reaction other than primers and template. It also contains loading dye solution including tracking dye (Bromophenol blue) for convenient post-PCR gel electrophoresis analysis. The MAGTaq 5X Blood Direct PCR Master Mix Kit is capable of high tolerance in the presence of PCR interfering/inhibiting substances in blood and is ideal for high through-put screening of blood sample for high reproducibility. The MAGTaq 5X Blood Direct PCR Master Mix Kit includes a pair of positive control primers (CCR5) that are compatible with primate blood sample.
Storage Buffer
5× Master Mix buffer, dNTP, Blood Direct DNA polymerase, Orange G and glycerol
Storage
4°C ≥ 6 months
-20°C ≥ 24 months
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Fig. 1. The MagTaq Blood Direct PCR Master Mix
amplified 200 bp from differently treated blood using CCR5 specific primers. M: MAGSPIN-21 marker.
Lane 1: fresh blood
Lane 2: blood +citrate
Lane3: blood +EDTA
Lane 4: blood+EDTA/NaF
Lane 5: blood+Heparin
Lane 6: 1mm2 of dry blood on filter paper
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