GEL/PCR Purification Mini Kit

MAGSPIN-86 96-well GEL/ PCR Clean Up kit is designed for rapid purification of fragment DNA from agarose gel PCR or other enzymatic reaction. The procedure uses chaotropic salt to dissolve agarose and denature enzyme. With the suitable binding condition provided by this system, the DNA in the sample mixture binds to glass fiber matrix in the 96-well DNA Binding Plate. The contaminants are washed with an ethanol-contained wash buffer and finally the purified DNA is eluted by low salt Elution buffer or water. The protocol does not require phenol extraction and alcohol precipitation. The entire procedure can be done within 30~40 minutes and the purified DNA is ready for restriction digestion, ligation, labeling, PCR, and sequencing reaction.

Specification:

     Sample Size: up to 50 μl PCR or other enzymatic reaction mixture, Up to 100 mg agarose gel slice
     Binding Capacity: up to 20 μg/ well
     DNA Size range: 70 bp~12Kb
     Operation: centrifuge/ vacuum manifold Handling Time: about 30 minutes for PCR clean up , about 40 minutes for gel             DNA extraction
     Recovery: 70% ~ 85% for Gel extraction , 90% ~ 95% for PCR clean-up
     Downstream application: Fluorescent or radioactive, sequencing, Restriction digestion, Library, screening,
        Ligation, Labeling, Transformation