96-well Genomic DNA Extraction Kit

96-Well Genomic DNA Extraction Kit is designed for high-throughput purification of total DNA (including genomic, mitochondrial and viral DNA) from whole blood and a variety of animal tissues or cells. The method use proteinase K and a chaotropic salt, guanidine hydrochloride to lyse cells and degrade protein, then DNA in chaotropic salt is bonded to glass fiber matrix of plate. After washing off the contaminants, the purified DNA is eluted by low salt elution buffer or water. The entire procedure can be completed in one hour without phenol/ chloroform extraction and alcohol precipitation. The kits can be used for manual filtration or with robotic handing systems and purified DNA with approximately 20-30 kb is suitable for PCR or other enzymatic reactions.

Specification:

     Sample Size: up to 200 µl of fresh/ frozen blood per well, up to 25 mg of animal tissue, up to 5 X 107 animal cultured cells         up to 108 bacterial cultured cells
     Binding Capacity: up to 30 μg/ well
     Elution Volume: 50-100 μl
     Operation: centrifuge/ vacuum manifold Handling Time: within 90 minutes