MAGFluoroStain Protein Fluorescent Staining Dye

(Gold, 100X)

Cat. No.                     Size
MAGSPIN-69            1 ml

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MAGFluoroStain Protein Fluorescent Staining Dye (Gold, 100X)

The MAGFluoroStain Protein Fluorescent Staining Dye (Gold, 100×) is designed to substitute the common Coomassie Blue protein staining method, offering greater sensitivity and ease of operation, compatible with visualization of proteins separated by 1-D or 2-D SDS-PAGE. MAGFluoroStain Protein Fluorescent Staining Dye (Gold, 100×) is normally low fluorescent but emits strong fluorescence (bright golden color) as bound to proteins. The staining procedure is a simple two-step protocol (fix and stain) that can be completed in as little as 30 minutes. Gels to be stained are fixed with ethanol/acetic acid solution prior to staining with MAGFluoroStain Protein Fluorescent Staining Dye (Gold, 100×) solution. A destain step is not recommended, but may be employed to reduce background, simply by agitating the gel in water for 1-5 minutes.

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Gels stained with MAGFluoroStain Protein Fluorescent Staining Dye (Gold, 100×) may be directly visualized with a variety of different UV-based fluorescence imaging systems. The maximum emission wavelength of protein-bound MAGFluoroStain Protein Fluorescent Staining Dye (Gold, 100×) is near 570 nm. MAGFluoroStain Protein Fluorescent Staining Dye (Gold, 100×) gives exceptional sensitivity and wide dynamic range for protein detection. The bound MAGFluoroStain Protein Fluorescent Staining Dye (Gold, 100×) is easily removed from the protein by immersing the gel in sufficient water, thus it is well compatible with subsequent enzymatic digestion and mass spectrometry for proteomics applications. Stained gels may be stored in stain solution in the dark at 2-8°C; imaging sensitivity might be moderately enhanced after 4°C storage of the stained gel.

Blood Genomic DNA Extraction MAXI Kit

MAGSPIN-60 Genomic DNA Extraction Maxi Kit is an excellent tool offering a speedy and economic method to purify total DNA (e.g. genomic, mitochondrial and viral DNA) from whole blood (fresh or frozen), plasma, serum, buffy coat, body fluids, lymphocytes and cultured cells. This technology first lyses cells and degrades protein by using a chaotropic salt and Proteinase K, then binds DNA to silica-based membranes, washes DNA with ethanol-contained Wash Buffer and then elutes purified DNA by low salt Elution Buffer or ddH2O. Compare with other harmful and timeconsuming procedures, such as phenol/chloroform extraction and ethanol precipitation, MAGSPIN-60 shortens the handling time about 1 hour. The size of purified DNA is up to 50 Kb (predominantly 20-30 Kb). After using MAGSPIN-60 Genomic DNA Extraction Maxi Kit, the high quality total DNA can be used directly for the downstream applications.

Specification:

MAGFluoroStain Protein Fluorescent Staining Dye (Gold, 100×) has its excitation peaks at 330 and 390 nm and emission maximum at 570 nm, making it compatible with UV-based imagers.

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