Blood Genomic DNA Extraction MAXI Kit

Cat. No.                     Size
MAGSPIN-60             10r

project

Blood Genomic DNA Extraction MAXI Kit

MAGSPIN-60 Genomic DNA Extraction Maxi Kit is an excellent tool offering a speedy and economic method to purify total DNA (e.g. genomic, mitochondrial and viral DNA) from whole blood (fresh or frozen), plasma, serum, buffy coat, body fluids, lymphocytes and cultured cells. This technology first lyses cells and degrades protein by using a chaotropic salt and Proteinase K, then binds DNA to silica-based membranes, washes DNA with ethanol-contained Wash Buffer and then elutes purified DNA by low salt Elution Buffer or ddH2O. Compare with other harmful and timeconsuming procedures, such as phenol/chloroform extraction and ethanol precipitation, MAGSPIN-60 shortens the handling time about 1 hour. The size of purified DNA is up to 50 Kb (predominantly 20-30 Kb). After using MAGSPIN-60 Genomic DNA Extraction Maxi Kit, the high quality total DNA can be used directly for the downstream applications.

Specification:

     Sample Size :up to 10 ml of fresh/ frozen blood, up to 1 x 10 of cultured cells
     Column Capacity: up to 500 µg of Genomic DNA
     Average DNA yield : 35 µg/ 1 ml whole blood
     Format: spin column
     Handling Time: about 1 hour
     Elution Volume: 1-2 ml