Total RNA Purification Kit
The Total RNA Purification Kit provides a rapid, simple and effective approach to isolate the total RNA from various animal/plant tissues, cultured cells and bacteria. The process is based on a spin column format. The procedure involves tissue or cells disruption and homogenization in the presence of guanidinium thiocyanate. After adding ethanol, RNA binds selectively to a silica membrane. Residual contaminating genomic DNA is digested by DNase I, which is applied directly in the column. After a series of rapid “wash-and-spin” steps to remove contaminating cellular components, the RNA remains bound to the membrane. Finally, nuclease-free water is used to elute the RNA from the membrane. Note that RNA shorter than 200 nt, such as 5S RNA, tRNA and microRNA, do not recover efficiently with this system.
- Features
Sample: bacteria(109), animal tissue(30 mg), cell culture(107), blood(0.5 ml fresh blood).
Purification format: silica membrane spin column.
RNA product: 10~100 μg /reaction.
Simple on-column DNase I treatment for genomic DNA removal.
No phenol/chloroform and ethanol precipitation needed.
- Experimental Data
RT-PCR of zebrafish EF-1α APS.
RNA were purified using kits from APS LABS and Q supplier. In the control reaction with only Taq polymerase
added to the RNA products,
large amount of genomic DNA contamination was observed for RNA purified using
RNA purification kit from supplier Q.
Non-denaturing gel electrophoresis of the total RNA purified from various samples using RNA purification kits from APS LABS and supplier Q.
General Procedure
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