Midi Plant Genomic DNA Purification Kit
The Midi Plant Genomic DNA Purification Kit provides a rapid, simple and effective approach to isolate the genomic DNA from plant tissue. The process is based on a modified salt precipitation method, the procedures involve cell lysis at 65°C, precipitation of proteins, polysaccharides and secondary metabolites, removal of cell debris by filter paper, and DNA precipitation by isopropanol. Up to 1 g plant tissue can be processed and DNA purified with this kit is suitable for various applications, including PCR, restriction enzyme digestion, cloning, dot blot analysis, etc.
- Features
Faster than traditional methods, purification can be done within 2 hours.
Specially designed for plant use: ssamples include general plants, alga, fungi, chinese medicine or
infected plant viral DNA.
Special formula for polysaccharides removal to increase DNA purity.
1g of sample can purify 30~500 μg of DNA product.
High Purity: DNA purified is ready to use for subsequent applications such as cloning, PCR amplification, enzyme
digestion, hybridization and sequencing.
Safe and Easy: No phenol/chloroform, no spin column.
- Experimental Data
Purification of plant genomic DNA from rice leaves usinf Midi Plant Genomic DNA Purification Kit
(1% of purified DNA were loaded on 1% agarose gel.)
Lane 1: 1 g of young rice leaf by Midi Plant Genomic DNA kit
Lane 2: 100 mg of young rice leaf by spin column kit from supplier A
Lane 3: 100 mg of young rice leaf by spin column kit from supplier B
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