MAGFluoroStain Protein Fluorescent Staining Dye (Red, 1000x)
The MAGFluoroStain Protein Fluorescent Staining Dye (Red, 1000×) is designed to substitute the common
Coomassie Blue protein staining method, offering greater sensitivity and ease of operation. Unlike Coomassie Blue stain,
the MAGFluoroStain Protein Fluorescent Staining Dye binds to protein with high specificity, making destaining process an option
rather than a requirement. With further reduction of background signals via destaining process, the MAGFluoroStain is capable of
achieving detection level parallel to silver staining without specialized imaging equipment (Fig. 1), making it one of the most
sensitive dyes available. In addition to its remarkable sensitivity, the MAGFluoroStain Protein Fluorescent Staining Dye (Red, 1000×)
brings a more reliable and safer user experience, since the stained gel can be visualized with blue-light illumination, avoiding
the risk of skin/ eye damage caused by UV light. For best result, we suggest using Lumix-Box™ Blue Light LED epi-illuminator to visualize
and analyze the gel stained with MAGFluoroStain Protein Fluorescent Staining Dye (Red, 1000×).
The MAGFluoroStain Protein Fluorescent Staining Dye is compatible to the analysis of mass spectra (Fig. 2), i.e. LC-MS/MS, MALDI-TOF, etc.
Fig.1. Comparison of MAGFluoroStain Protein Fluorescent Staining Dye (Red, 1000×) with Silver stain of a 2× serially diluted BSA sample.
Fig.3. Excitation and emission spectrum of FluoroStain Protein Fluorescent Staining Dye (Red, 1000×).
Storage
Store at -20°C for 12 month
Fig.2. Compariosn of MALDI-TOF mass spectra of bovine serum albumin (BSA) stained with MAGFluoroStain Protein Fluorescent Staining Dye (A) and with Coomassie Blue (B). BSA proteins are seperated on SDS-PAGE, stained with fluorescent dye or conventional Coomassie Blue, followed by trypsin digestion in gel, and then by MALDI-TOF
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