Plasmid Miniprep Purification Kit II
TThe Plasmid Miniprep Purification Kit II provides protocol and materials for a spin column-based purification of high quality plasmid DNA from 1~10 ml liquid culture of both EndA+ and EndA strains of Escherichia coli. High quality DNA without RNA contamination can be prepared within 10~15 min. The DNA yield of up to 40 μg is quantitative and reproducible, and can be used for many applications, including polymerase chain reaction, restriction digestion, cloning, DNA sequencing, hybridization and in vitro transcription.
- Features
Easy to operate: Spin Column format
High Yield: Can purify 40 μgultra pure plasmid DNA from 1~5 ml bacterialculture.
High Purity: High purity plasmid DNA that can be used directly forautomatic sequencing, in vitro transcription, PCRand
enzyme digestion or any other related experiments
Fast:The procedures can be done in 10~15 minutes.
Exclusive formula:Effective removal of endonuclease, especially effective for EndA+ bacteria, L21(DE3), HB101 and JM109.
General Procedure
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