Plasmid Midiprep Purification Kit
The Plasmid Midiprep Purification Kit provides simple rapid protocol and materials to purify DNA from 20~100 ml bacteria liquid culture. The yield of DNA, from 30 μg~300 μg, is quantitative and reproducible. The obtained DNA can be used for many applications, including PCR, restriction enzyme digestion, cloning, automated DNA sequencing, southern blot, in vitro transcription assay, and transfection.
- Features
Easy to operate: Spin Column format, similar to miniprep.
High purity: Plasmid DNA purified with removal of endotoxins, and can perform transfectionand automatic sequencing.
High Yield: Each reaction is capable of purifying 100~300 µg plasmid DNA from 20~100 ml bacterial suspension.
Fast:The whole process can be done within an hour.
Supplier | Final concentration | A260/A280 | Yield | Average |
Supplier Q | 606 ng/µl | 1.9 | 181.8 µg | 184.6 µg |
624 ng/µl | 1.89 | 187.2 µg | ||
616 ng/µl | 1.87 | 184.8 µg | ||
APS LABS |
708 ng/μl | 1.91 | 212.4 μg | 211.1 μg |
910 ng/μl | 1.91 | 273.0 μg | ||
692 ng/μl | 1.91 | 207.6 μg | ||
638 ng/μl | 1.91 | 191.4 μg |
General Procedure
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