PCR Hot-Start Master Mix II (5X)-(final 25ul)

The PCR Hot-Start Master Mix II (5X) is a ready-to-use mixture for virtually all PCR applications. Hot Start Taq DNA polymerase is a complex mixture of thermostable Taq DNA polymerase and specific monoclonal antibodies. It can eliminate amplification artifacts such as primer-dimer formation and mis-priming during preamplification stage and thus may provide improved specificity when compared to the standard DNA polymerase. The mixes contain Hot-Start Taq DNA polymerase and other components for PCR with the exception of template and primers. This not only saves valuable time in the laboratory, but also reduces the number of pipetting and reagent handling errors. The PCR Hot-Start Master Mix II (5X) is supplied as a 5X concentrated ready-to-use mix containing 0.75 u of Hot Start Taq DNA polymerase, reaction buffer, MgCl2 ( final concentration 2 mM), 250 μM dNTP and enzyme stabilizer in a 25 μl 1X PCR mixture. Store at -20°C.