PCR Dye Hot-Start Master Mix (5X)

The PCR Dye Hot-Start Master Mix (5X) is a ready-to-use mixture for virtually all PCR applications. Hot-Start Taq DNA polymerase is a complex mixture of thermostable Taq DNA polymerase and specific monoclonal antibodies. It can eliminate amplification artifacts such as primer-dimer formation and mis-priming during preamplification stage and thus may provide improved specificity when compared to the standard DNA polymerase. The mixes contain Hot-Start Taq DNA polymerase and other components for PCR with the exception of template and primers. A red dye containing in the mix allow the PCR product to be direct loaded on the gel. This not only saves valuable time in the laboratory, but also reduces the number of pipetting and reagent handling errors. The PCR Dye Hot-Start Master Mix II (5X) is supplied as a 5X concentrated ready-to-use mix containing 0.75 u of Hot-Start Taq DNA polymerase, reaction buffer, 2 mM MgCl2, 250 μM dNTP and enzyme in a 25 μl 1X PCR mixture. Store at -20°C.