Two-Step AMV RT-PCR kit
The APSLABS Two-Step AMV RT-PCR Kit has all the components required to perform first-strand cDNA synthesis and second strand DNA amplification. Avian Myeloblastosis Virus Reverse Transcriptase (AMV RTase) can catalyze polymerization of DNA using DNA, RNA or DNA:RNA hybrids as templates. Besides possessing 5’-3’ DNA polymerase activity, the enzyme also possess some RNaseH activity, which breaks apart RNA:DNA hybrids. It is used primarily for the synthesis of first and second strand complementary DNA (cDNA) and primer extensions. In the cDNA synthesis step, RNA was reverse transcribed by AMV RTase to produce its cDNA. For the subsequent amplification of the cDNA template, PCR Hot-Start Master Mix II is provided. It can eliminate amplification artifacts such as primer-dimer formation and mis- priming during preamplification stage and thus may provide improved specificity. PCR Hot-Start Master Mix II can be used for effective amplification of DNA up to 6 kb in length.
- Features
• Purpose: Reverse transcription of RNA templates into first strand cDNA
• Enzyme Properties: Contains AMV reverse transcriptase and Hot-start PCR mix, with high efficiency and specificity. Is able to
synthesize DNA at 42℃ (more efficient than M-MLV)
• Primer: Oligo(dT)18 or Random hexamerprimer available for choosing according to needs (can also choose self-designed
specific primers)
• Applications 2nd strand cDNA synthesis, PCR, cloning etc
- Experimental Data
Figure 1. RT-PCR products of E.colitotal RNA generated using Two-step AMV RT-PCR Kit. E.Coli(DH5α) total RNA is used as template to generate cDNA. E.coli fruK gene is further amplified using the same kit.
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