One-Step RT-PCR Plus Kit(MAGscript/MAGTaq Plus)

The One-Step RT-PCR Plus Kit (MAGscript RT/MAGTaq Plus) is designed for the reverse transcription and PCR amplification of a specific target RNA from either total RNA or mRNA. This one-tube, two-enzyme system provides sensitive, quick and reproducible analysis of even rare RNAs. The system uses MAGscript reverse transcriptase for first-strand cDNA synthesis and MAGTaq Plus™ for PCR amplification. MAGscript Reverse Transcriptase (MAGscript RTase) is genetically engineered version of Moloney Murine Leukemia Virus Reverse Transcriptase (M-MLV RTase), which lacks a 3’ to 5’ exonucleolytic proofreading function and has a weak RNase H activity as compared to AMV reverse transcriptase, and is useful for full-length cDNA amplification. The kit contains MAGTaq Plus™, which is an enzyme mixture of MAGTaq and Pfu DNA polymerase. It has high fidelity with an error frequency of 1.6/106 during DNA synthesis, and the PCR product can be directly used for cloning to TA or expressional vectors.