APSMAGTaq 5X Fluorescent PCR Master Dye Mix
The MAGTaq 5× Fluorescent PCR Master Mix is a ready-to-use mixture for amplifying targeted DNA fragments. It is designed to serve as ready-to-use master mix for virtually all PCR applications. The MAGTaq 5× Fluorescent PCR Master Mix is supplied as a 5× concentrated ready-to-use mixture containing all the essential ingredients for PCR with the exception of template and primers. In addition, the mixture contains electrophoresis tracking dye (Bromophenol blue), as well as a safer fluorescent DNA staining dye which enables the user to track the electrophoresis process in real time as well as eliminating the need for staining process. The resultant PCR reaction mixture is sufficiently dense to be loaded directly into 1×TAE or 1×TBE buffer for electrophoresis.
- Features
• 5'→3' DNA polymerase activity Fig. 1. λDNA was amplified with MAGTaq 5X Fluorescent PCR Master Mix. The λDNA was amplified with specific primers for amplifying different regions. (M: MAGSPIN-119 marker)
• None detectable 3'→5' exonuclease (proofreading) activity
• Generates PCR products with 3'-dA overhangs
• High throughput PCR
• High yield PCR
• High reproducibility, less pipetting error
• Loading directly into electrophoresis
• DNA band can be visualised directly under UV or 470 nm blue light illumination
- Component
Volume
MAGTaq 5× Fluorescent PCR Master Mix 1 ml × 2
Storage
4°C ≥ 6 months
-20°C ≥ 24 months
Fig. 1. λDNA was amplified with MAGTaq 5X Fluorescent PCR Master Mix. The λDNA was amplified with specific primers for amplifying different regions. (M: MAGSPIN-119 marker)
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