MAGTaq DNA Polymerase
MAGTaq DNA Polymerase is a recombinant thermo-stable DNA polymerase expressed and purified from an E. coli strain carrying the cloned MAG. With high DNA synthesis rate and thermo-stability, MAGTaq DNA Polymerase is suitable for general and specialized PCR applications
- Features
• 5'→3' DNA polymerase activity Fig. 1. MAGTaq DNA Polymerase demonstrated superior yield across a wide range of template concentration
compared to other commercially available Taq polymerase..
• 5'→3' exonuclease activity
• None detectable 3'→5' exonuclease (proofreading) activity
• Generates PCR products with 3’-dA overhangs
• Thermo-stable-half life is more than 40 min at 95°C
- Component
Volume
MAGTaq DNA Polymerase 100 μl
10× Reaction Buffer 1 ml × 2
MAGTaq DNA Polymerase
DNA polymerase 5 units/ µl
Storage
20 mM Tris-HCl (pH 8.0), 100 mM KCl, 0.1 mM EDTA, 1 mM DTT, stabilizer, 50% (v/v) glycerol
10X Reaction Buffer
200 mM Tris-HCl (pH 8.8 at 25°C), 100 mM KCl, 100 mM (NH4)2SO4, 20 mM MgCl2, 1% Triton X-100
Unit Definition
One unit is defined as the amount of enzyme that will incorporate 10 nmol of dNTP into acid-insoluble material in 30 minutes at 74°C.
Storage
-20°C ≥ 24 months
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