APSMAGTaq SEMI-Taq DNA Polymerase
MAGTaq SEMI-Taq DNA Polymerase is a specially blended enzyme mix containing KlenTaq-1 DNA polymerase (a 5’-exo-minus, N-terminal deletion of Taq DNA polymerase) and a small amount of a proofreading DNA polymerase. This unique blending helps to improve the fidelity, yield and processivity of the resultant PCR process. The SEMI-Taq is also highly robust, showing high tolerance of varying concentration of Mg2+; it is highly thermostable and has four times the fidelity compared to MAGTaq DNA polymerase. The MAGTaq SEMI-Taq DNA Polymerase is ideal for DNA amplifications 0.5-5 kb in length on genomic DNA, and up to 10 kb on less complex templates.
MAGTaq SEMI-Taq DNA Polymerase mixture
DNA polymerase 5 units/ µl
A proofreading DNA polymerase Trace
Storage Buffer
40 mM Tris-HCl (pH 7.5), 50 mM KCl, 25 mM (NH4)2SO4, 0.1 mM EDTA, 5.0 mM 2-mercaptoethanol, 50% (v/v) glycerol
10X Reaction Buffer
400 mM Tricine-KOH (pH 9.2 at 25°C), 150 mM KOAc, 35 mM Mg(OAc)2, 750 µg/ ml Bovine Serum Albumin Unit Definition One unit is defined as the amount of enzyme that will incorporate 10 nmol of dNTP into acid-insoluble material in 30 minutes at 74°C.
Storage
-20°C ≥ 24 months
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