APSMAGTaq Hifi DNA Polymerase
The MAGTaq HiFi DNA Polymerase is a new genetic modified, recombinant DNA polymerase with 70 times higher fidelity during amplification than MAGTaq DNA polymerase, as well as very high elongation rate. Being highly thermostable, the HiFi DNA Polymerase can remain viable even after being subjected to boiling for 2 minutes. The MAGTaq HiFi DNA Polymerase is also designed to operate in much lower Mg2+ concentration as compared to other DNA polymerase.
- Features
• 5'→3' DNA polymerase activity Fig.1. High yield of HiFi DNA Polymerase in PCR reaction.(M: MAGSPIN-21 marker)
• 3'→5' exonuclease activity (proofreading)
• High reaction rate (up to 67 base/ second)
• High fidelity, 70 times of Taq polymerase q
• Blunt end amplicon
• Thermo-stable-half life is more than 10 hrs at 95°C
- Component
Volume
MAGTaq HiFi DNA Polymerase 100 μl
10× Reaction Buffer 600 μl
25 mM MgSO4
500 μl
dNTPs Mix (2 mM each) 600 μl
DMSO
600 μl
MAGTaq HiFi DNA Polymerase mixture
HiFi DNA Polymerase 1 units/ µl
Storage
-20°C ≥ 12 months
Storage Buffer
50 mM Tris-HCl (pH 8.0), 50 mM KCl, 0.1 mM EDTA, 1 mM DTT, stabilizers, and 50% (v/v) glycerol
Unit Definition
One unit of HiFi DNA Polymerase incorporates 10 nmol of deoxyribonucleotide into acid-insoluble material in material in 30 min at 74°C.
Storage
-20°C ≥ 24 months
Fig.2.HiFi DNA Polymerase performs well for high GC content Rhodobactor gDNA templates, with annealing temperature ranging from 59 to 63°C.
- DOWNLOAD PROTOCOL